Effects of extracorporeal shock wave therapy on interleukin-1beta and matrix metalloproteinase-13 expression in rabbits with knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2014.15.017

Abstract

Abstract:

BACKGROUND: Interleukin-1β and matrix metalloproteinase-13 can promote the metabolism of chondrocytes, inhibit the capacity of synthesizing and repairing, induce the degradation of extracellular matrix, and play a crucial role in the occurrence of osteoarthritis.
OBJECTIVE: To observe the effects of extracorporeal shock wave therapy on the interleukin-1β and matrix metalloproteinase-13 expression in rabbits with experimental knee osteoarthritis.
METHODS: Thirty New Zealand rabbits were randomly and equally divided into treatment group, model group and control group, with 10 rabbits in each group. Model of knee osteoarthritis was established in both the treatment group and model group, using modified plaster cast in extension position for 6 weeks. Then the rabbits of treatment group were treated with extracorporeal shock wave therapy, each 1 000 impulse, at the energy flux density of 0.1 mJ/mm². There were no treatments in the control group. The rabbits in each group were sacrificed at 4 weeks after treatment, the knee synovial fluid and articular cartilage were collected from the rabbits. The pathological changes of knee joint were detected using hematoxylin-eosin staining and toluidine blue staining. The interleukin-1β and matrix metalloproteinase-13 expression in the synovia was detected using ELISA and immunohistochemical staining respectively.
RESULTS AND CONCLUSION: The interleukin-1β concentration in the synovial fluid was significantly higher in the treatment group and model group than the control group (P < 0.01), and the treatment group after treatment showed a lower concentration than the model group (P < 0.05). Mankin scores in treatment group and model group were significantly increased compared with the control group (P < 0.01), and the treatment group after treatment showed a lower score than the model group (P < 0.05). The interleukin-1β and matrix metalloproteinase-13 positive expression rates in the treatment group and model group were significantly increased compared with the control group (P < 0.01), and the treatment group after treatment showed a lower rate than the model group (P < 0.05). The extracorporeal shock wave therapy can downregulate the expression of interleukin-1β and matrix metalloproteinase-13, promote the synthesis of new collagen Ⅱ and proteoglycan, therefore effectively improve the osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: low-energy shock waves, osteoarthritis, knee, interleukin-1beta, matrix metalloproteinase-13

CLC Number:

R318

Hou Xiao-dong, Liu Hong-bo, Liu Ke-min. Effects of extracorporeal shock wave therapy on interleukin-1beta and matrix metalloproteinase-13 expression in rabbits with knee osteoarthritis [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(15): 2397-2402.

Figures/Tables 3

2.1 实验动物数量分析参加实验数量30只实验兔，进入结果分析30只实验兔，中途无脱落。 2.2 软骨病理学观察对照组：软骨表面光滑，表层细胞呈梭形，近似水平排列，中间层细胞圆形，散在分布，柱状层细胞排列呈柱状，软骨4层结构及潮线清晰可见，基质染色无减退。模型组：软骨层变薄，软骨细胞数目减少，细胞排列紊乱，基质染色明显减退，潮线不完整。治疗组：软骨层变薄，细胞排列紊乱，基质染色略减退，潮线不清。对照组软骨组织Mankin评分为0.63±0.51，模型组软骨组织Mankin评分为10.01±1.94，治疗组软骨组织Mankin评分为7.83±1.39。治疗组和模型组的Mankin评分均高于对照组，差异有非常显著性意义(t=12.114，15.204，P < 0.01)；治疗组Mankin评分明显低于模型组，差异有显著性意义(t=2.433，P=0.038 < 0.05)。 2.3 兔关节液中白细胞介素1β水平对照组关节液中白细胞介素1β水平为(33.60±2.32) ng/L，模型组关节液中白细胞介素1β水平为(72.91±8.94) ng/L，治疗组关节液中白细胞介素1β水平为(54.44±5.14) ng/L。治疗组和模型组兔膝关节液中白细胞介素1β水平较对照组明显升高(t=11.215，14.071，P < 0.01)，而体外冲击波干预治疗后，治疗组白细胞介素1β水平较模型组有下降(t=4.522，P=0.01)。 2.4 免疫组织化学染色结果对照组软骨细胞胞浆中偶见浅棕黄色白细胞介素1β、基质金属蛋白酶13弱阳性染色，且主要在浅层软骨细胞内(图1A，2A)。模型组见大量软骨细胞呈深棕色白细胞介素1β、基质金属蛋白酶13强阳性染色颗粒(图1B，2B)。治疗组软骨细胞胞浆呈浅棕黄色白细胞介素1β、基质金属蛋白酶13阳性染色(图1C，2C)。从表1可以看出，模型组和治疗组白细胞介素1β、基质金属蛋白酶13的表达都较对照组上调，差异均有显著性意义(P < 0.01)。治疗组的表达较模型组下调，差异有显著性意义(P < 0.05)。"

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